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In vitro prenylation reactions analyzed by β-CD-Sepharose FPLC.
| Protein | Enzyme | Diphosphate Substrate | Retention Time (min) |
|
|---|---|---|---|---|
| Unmodified Protein | Prenylated Protein | |||
| mCherry-CVIA | PFTase | 1-PP | 4.7 | 41.2 |
| mCherry-CVIA | PFTase | 3-PP | 4.3 | 42.9 |
| eGFP-CVIA | PFTase | 3-PP | 8.7 | 42.1 |
| CDC42-CVLL | PGGTase-I | 2-PP | 5.7 | 35.6 |
| CDC42-CVLL | PGGTase-I | 4-PP | 4.8 | 35.0 |
| GST-CIIS | PFTase | 1-PP | 6.7 | 37.2 |
| H-Ras-CVLS | PFTase | 1-PP | 5.9 | 36.6 |
