Fig. 1.
TLX1 interacts with TLE1 in vitro via an Eh1-like motif. (A) TLX1 mutants. Abbreviations: ΔN, NH2-terminal deletion; ΔC, COOH-terminal deletion; ΔHD, homeodomain deletion; ΔH3, deletion of the third helix of the homeodomain. (B) GST-TLX1 pulldowns in 293T cells transiently transfected with a TLE1 expression vector (293T/TLE1). Due to the absence of an epitope recognized by the anti-TLX1 antibody, GST-TLX1ΔC70 and GST-TLX3 fusion proteins were detected by an anti-GST antibody (▹).