Table 3.

Summary the retrospective analysis of the qHTS

9.2 uM	Threshold	TP	FP	FN	TN
Class 1–2	3 σ	50	129	7	9,735
	6 σ	38	31	19	9,833
Class 1–3	3 σ	76	103	NA	9,668
	6 σ	45	24	NA	9,747
1.8 uM	Threshold	TP	FP	FN	TN
Class 1–2	3 σ	40	131	NA	9,733
	6 σ	31	27	NA	9,837

TP, true positive; FP, false positive; FN, false negative; TN, true negative. NA, not applicable. Class 3 CR curves are relevant for this analysis when the 1.8 uM or 9 uM was the highest tested concentration as these curves are fit to a single point. However, all libraries were screened at a higher concentration then 1.8 uM and some were screened at higher than 9 uM and therefore the false negative numbers are found to be inflated when including this curve class. Therefore, for calculation of false negatives we only report the numbers using the high confidence curve classes. Also, note for this reason the TP and FP numbers are the same using Class 1–3 curve class as the comparison set for the 1.8 uM dataset.