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Western bolt and flow cytometric analysis for the antigen of McAb4E7. (A) Western bolt detection for the expression of the antigen of McAb4E7, lane1: H-128 cells; lane 2: A549 cells; lane 3: MRC-5 cells; GAPDH was used as reference, the expression of the antigen of McAb4E7 was found in A549 cells (B-D) Flow cytometry analysis for the localization of the targeting antigen, the localization of antigen of McAb4E7 was in cellular membrane of A549 cells, but not in MRC-5 and H-128 cells.
