Table 1.
Diet Formulations
| Diet Ingredientsa | ZD | C | ZS |
|---|---|---|---|
| (g/kg diet) | (3 mg Zn/kg diet) | (30 mg Zn/kg diet) | (300 mg Zn/kg diet) |
| Dextrose (cerelose) | 603.8 | 594.6 | 504.6 |
| Egg white | 212.5 | 212.5 | 212.5 |
| Fiber (cellulose) | 50 | 50 | 50 |
| Mineral mix (AIN-93M-MX Zn-free) | 35 | 35 | 35 |
| Potassium phosphateb | 5.4 | 5.4 | 5.4 |
| Vitamin mix (AIN-93-VX) | 10 | 10 | 10 |
| Choline | 2.5 | 2.5 | 2.5 |
| Biotin mixc | 10 | 10 | 10 |
| Zn premixd | 0.8 | 10 | 100 |
| Soybean oil | 70 | 70 | 70 |
aDiet ingredients were obtained from Harlan Teklad, except the dextrose (Moonshiners) and potassium phosphate (Fisher Scientific)
bAdditional potassium phosphate was added to make the AIN-93M-MX mineral mix equivalent to the AIN-93G-MX mineral mix
c200 mg biotin/kg dextrose; additional biotin due to avidin in egg white, and egg white as the protein source to formulate zinc deficient diet
d5.775 g zinc carbonate/kg dextrose