Fig. 5.

Western blotting analysis of expression of iNOS in the colon. At the indicated times after BDL, colon specimens were collected. Each amounts of protein (30 µg/lane) from colon was subjected to a 7.5% SDS-PAGE, and the electrophoresed proiteins were transferred onto Immobilon-P membranes, and analyzed using anti-iNOS antibody. The bands with 130 kDa responsible for iNOS were seen in colon specimens from BDL mice. Data show one typical result out of 3 representative experiments.