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Treatment with ICI 182,780 does not block the E2-induced LHRH release. E2 at 1 nm or vehicle was infused for 20 min in the presence of ICI 182,780 at 100 nm. Note that E2 with ICI 182,780 resulted in an increase in LHRH release, whereas vehicle with ICI 182,780 did not cause any significant effect. *, P < 0.05 vs. before E2; a, P < 0.05 vs. vehicle.
