Figure 5.
A, The presence of GPR30 cDNA products were identified by RT-PCR in olfactory placode cultures, and samples were obtained from the uterus, cerebellum, and hypothalamus in female rhesus monkeys. GPR30 amplicons (∼600 bp) in olfactory placode cultures were cloned and sequenced for identification of the monkey GPR30 cDNA. H2O is control. B, Colocalization of GPR30 in LHRH neurons. Immunopositive LHRH neurons in the medial basal hypothalamus are shown in green (a and d), and immunopositive GPR30 neurons are shown in red (b). IgG does not cause any neuronal staining (e). Overlay pictures (c and f) show that LHRH neurons are double stained with GPR30 (yellow), but non-LHRH neurons (arrowheads) express only GPR30.