Figure 2.

GnRH-stimulated increases in Jun expression are reduced in TCFDN-expressing LβT2 cells. LβT2 cells were transduced with either GFP- or TCFDN-expressing adenoviruses (1 × 10¹¹ vpm) and subsequently treated with either vehicle or 100 nm GnRH for 1 h. A, Representative endpoint RT-PCR experiment using primers that span either the DNA-binding domain of TCF7L2 or cyclophilin B. B, Whole-cell extracts were isolated from GFP- and TCFDN-transduced LβT2 cells and subjected to Western blot analysis using antibodies specific for TCF7L2. Quantitative real-time PCR was used to determine relative levels of Jun (C), Atf3 (D), or Egr1 (E) expression. Levels of cyclophilin expression were used to normalize samples. The expression levels of each gene are expressed relative to the amount of expression in vehicle-treated GFP-expressing LβT2 cells. Data shown are the means ± sem from three distinct experiments in which each sample was assayed in triplicate. *, P < 0.05.