Figure 5.

CGA transcription is compromised by TCFDN. A, LβT2 cells were cotransfected with a human CGA-Luc promoter reporter (50 ng) and either an empty CMV expression vector (control) (100 ng) or a TCFDN expression vector (100 ng) and then treated with vehicle or 100 nm GnRH for 24 h. B, LβT2 cells were cotransfected with an Egr1-Luc promoter reporter (50 ng) and either an empty CMV expression vector (control) (100 ng) or a TCFDN expression vector (100 ng) and then treated with vehicle or GnRH for 24 h. Data shown are the means ± sem from three independent experiments performed in triplicate. *, P < 0.05.