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Altered lineage allocation in CTGF null pancreata. When compared with controls (A), CTGF null mutant embryos showed no difference in glucagon area (red) at e12.5 (D). However, an increase in glucagon+ cells was seen in CTGF mutant embryos beginning at e13.5 (compare E with B) and continuing throughout embryonic development. By e15.5. there was clearly a decrease in insulin+ cells (green) in the mutant pancreata (compare C and F). Magnification: × 200 (A, B, D, and E); × 400 (C and F).
