Figure 2.

The synthesis and secretion of representative clones expressing either CG or CGΔT dimers after labeling for 4 h with 20 μCi/ml [³⁵S]cysteine (A) or with 0.7 mCi/ml of [³⁵S]sulfate (B). Media (M) and lysates (L) were immunoprecipitated with anti-CGβ serum, resolved by SDS-PAGE, and subjected to autoradiography. The migration of α- and β-subunits, and the molecular mass markers are indicated. β* represents the mature form of β-subunit. Note that α-subunit comigrates with the mature form of CGβΔT. The data shown are representative of five independent experiments.