Figure 3.
Pulse-chase kinetics of CG (A and C) and CGΔT (B) dimers. GH3 cells were pulse-labeled with 80 μCi/ml [35S]cysteine for 20 min and then chased for the indicated times. Chase at 0 h indicates the lysate sample prepared immediately after the pulse. Lysates and media were immunoprecipitated with CGβ antiserum and subjected to SDS-PAGE followed by autoradiography. The migration of α- and β-subunits is indicated. β* represents autoradiographs for CG that exposed the mature forms of β-subunit. The data shown are representative of three independent experiments. Note that exposure was for 16 (A) or 24 h (C) for CG and 16 h for CGΔT (B).