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. 2009 Jan 8;23(3):412–421. doi: 10.1210/me.2008-0235

Figure 5.

Figure 5

ATO disrupts androgen-mediated AR N-C interaction. PC3 cells were transfected with Gal4-LUC reporter and (A) control: Gal4-ARLBD + VP16-ARTAD + empty vector (EV); cyclin D: Gal4-ARLBD + VP16-ARTAD + cyclin D; Gal4-ARLBD: Gal4-ARLBD + EV; VP16-ARTAD: VP16-ARTAD + EV (B) Gal4-ARLBD + VP16-ARTAD + either EV Control or TIF2 or SRC3. All cells were treated with vehicle, 1 nm R1881, or 1 nm R1881 and 5 μm ATO for 24 h. Luciferase activity was determined 48 h after transfection. Means of triplicate samples are plotted ± sem. RLU, Relative luciferase units.