Figure 7.

ATO and bicalutamide treatment effectively inhibit AR transcriptional activity. LNCaP cells were transfected with a PSA (ARE containing)-luciferase reporter plasmid. Cells were treated with either vehicle or 1 nm R1881 in the presence or absence of 20 μm Casodex. The cells were also treated with either 0 or 2 μm ATO. Luciferase activity was determined 48 h after transfection and treatment using a luminometer. Means of triplicate samples are plotted ± sem. P values were determined by comparison of androgen treatment vs. androgen plus ATO or androgen plus bicalutamide, or all three treatments. *, P < 0.05; **, P < 0.01. RLU, Relative luciferase units.