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. 2009 Mar 20;4(3):e4870. doi: 10.1371/journal.pone.0004870

Figure 3. Specificity and efficiency of fluoromycobacteriophage infections.

Figure 3

A. A mixture of E. coli cells expressing DsRed-Express and M. smegmatis were infected with (a) phAE87::hsp60-EGFP or (b) mock infected. From left to right: fluorescence micrograph images using a ZsGreen1 filter (green); fluorescence micrograph images using a HQ:R NX filter (red), merged fluorescence images; phase contrast images. Red cells: E. coli; green cells: M. smegmatis mc2155 infected with pHAE87::hsp60-EGFP. Scale bar, 10 µm. B. M. smegmatis mc2155 cells were infected with phAE87::hsp60-EGFP at different multiplicities of infection (moi). Green cells were counted in 15 individual fields and the percentage of fluorescent cells compared to the total number of cells in phase contrast images determined.