Figure 10. Detection of M. tuberculosis mc²6230 Rif^R cells in a mixed population using flow cytometry.

M. tuberculosis mc²6230 wt was mixed with the isogenic rif^R strain at different proportions. A total of 10⁷ mixed cells were infected with phAE87::hsp60-EGFP in the presence of Rifampicin; cells were fixed and analyze by flow cytometry. (A) 100% wt: 0% Rif^R (B) 50% wt: 50% Rif^R; (C) 75% wt : 25% Rif^R ; (D) 90% wt :10% Rif^R; (E) 99% wt :1% Rif^R . Left panels, the intensity of fluorescence is plotted against light side scatter; right panels, the level of fluorescence is plotted against the number of events counted.