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. Author manuscript; available in PMC: 2010 Feb 18.
Published in final edited form as: Vaccine. 2009 Jan 4;27(8):1216–1229. doi: 10.1016/j.vaccine.2008.12.014

Figure 3.

Figure 3

Bacterial burden in the host tissues of mice after intranasal challenge with F. tularensis LVS. Forty BALB/c mice were anesthetized with Ketamine/Xylazine and then infected i.n. with 4400 CFU of LVS. At the indicated times post-infection, 8 mice per group were euthanized and the liver, spleen and lung were removed and homogenized in 2 ml of sterile PBS for 15 seconds in a Pro200 homogenizer. CFU of F. tularensis in each organ was determined by plating serial dilutions of the homogenates on Chocolate agar containing sulfamethoxazole (40 mg/ml), trimethoprim (8 mg/ml) and erythromycin (50 mg/ml) and culturing for 3 days at 37°C. Values represent the mean Log CFU per organ ± SE.