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. Author manuscript; available in PMC: 2010 Jan 1.
Published in final edited form as: J Invest Dermatol. 2008 Jun 26;129(1):162–166. doi: 10.1038/jid.2008.182

Figure 2. FSK promotes the removal of UVB-induced CPD and 64PP but is independent of pigmentation.

Figure 2

(a)Fontana-Masson staining of reconstructed skins exposed to 21 mJ/cm2 UVB and treated 30 min before UV exposure with or without 20 μM FSK. Skin specimens were fixed 6, 24 and 48 h after the UVB exposure. The mean (± SD, n=2) melanin content (in arbitrary units) is shown below each panel and the statistical significance from the untreated control is indicated (NS – not significant). (b) Immunostaining of reconstructed skins exposed to 21 mJ/cm2 UVB and treated for 30 min before exposure with or without 20 μM FSK. Skin specimens were fixed 5 min, 6 h, 24 h and 48 h after the UVB exposure. CPD (left) and 64PP (right) are stained red, nuclei in all images are stained blue with DAPI. The mean density (± SD, n=3) of CPD or 64PP damage per nucleus is shown below each panel and the statistical significance from the untreated control is indicated (NS = not significant). All images in each panel are at the same magnification; bars = 100 μm.