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Stability of sigma-32 in E. coli MPh42 cells. Pulse-chase and immunoprecipitation experiment was performed using 100 μCi/ml 35S-methionine and anti-sigma-32 antibody, as described in 'Methods'. Panel A: cells grown at 30°C in the presence of CCCP, panel B: control cells at 30°C, and panel C: cells submitted to 50°C. The numbers in the lanes signify the time of chasing in minutes.
