Skip to main content
NCBI home page
Journal of Clinical Microbiology logoLink to Journal of Clinical Microbiology
. 1992 Aug;30(8):2181–2183. doi: 10.1128/jcm.30.8.2181-2183.1992

Evaluation of a urease-based confirmatory enzyme-linked immunosorbent assay for diagnosis of Neisseria gonorrhoeae.

M Carballo 1, J R Dillon 1, M Lussier 1, P Milthorp 1, S Winston 1, B Brodeur 1
PMCID: PMC265467  PMID: 1500530

Abstract

A new urease-based enzyme-linked immunosorbent assay utilizing novel monoclonal antibodies was evaluated for the culture confirmation of Neisseria gonorrhoeae, with 270 isolates of N. gonorrhoeae, 56 isolates of diverse Neisseria spp., and 29 Moraxella isolates. The test was highly specific (100.00%) and sensitive (97.83%). No cross-reactions were observed with any of the Neisseria or Moraxella isolates tested. Fifty percent (3 of 6) of the false-negative results were obtained with isolates of serovar IA-4, a serovar rarely encountered in North America.

Full text

PDF
2181

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Arko R. J., Finley-Price K. G., Wong K. H., Johnson S. R., Reising G. Identification of problem Neisseria gonorrhoeae cultures by standard and experimental tests. J Clin Microbiol. 1982 Mar;15(3):435–438. doi: 10.1128/jcm.15.3.435-438.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Boehm D. M., Bernhardt M., Kurzynski T. A., Pennell D. R., Schell R. F. Evaluation of two commercial procedures for rapid identification of Neisseria gonorrhoeae using a reference panel of antigenically diverse gonococci. J Clin Microbiol. 1990 Sep;28(9):2099–2100. doi: 10.1128/jcm.28.9.2099-2100.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Brodeur B. R., Ashton F. E., Diena B. B. Enzyme-linked immunosorbent assays for the detection of Neisseria gonorrhoeae specific antibodies. Can J Microbiol. 1978 Nov;24(11):1300–1305. doi: 10.1139/m78-211. [DOI] [PubMed] [Google Scholar]
  4. Chandler H. M., Coulter A. R., Healey K., Kornitschuk M., MacGregor A., Hurrell J. G. Monoclonal hybridoma antibodies against human IgE and their use in a rapid and sensitive enzyme immunoassay for the semiquantitative assessment of total IgE levels in human blood. Int Arch Allergy Appl Immunol. 1983;72(3):267–272. doi: 10.1159/000234879. [DOI] [PubMed] [Google Scholar]
  5. Coghill D. V., Young H. Serological classification of Neisseria gonorrhoeae with monoclonal antibody coagglutination reagents. Genitourin Med. 1987 Aug;63(4):225–232. doi: 10.1136/sti.63.4.225. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Dillon J. R., Bygdeman S. M., Sandström E. G. Serological ecology of Neisseria gonorrhoeae (PPNG and non-PPNG) strains: Canadian perspective. Genitourin Med. 1987 Jun;63(3):160–168. doi: 10.1136/sti.63.3.160. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Dillon J. R., Carballo M., Pauzé M. Evaluation of eight methods for identification of pathogenic Neisseria species: Neisseria-Kwik, RIM-N, Gonobio-Test, Minitek, Gonochek II, GonoGen, Phadebact Monoclonal GC OMNI Test, and Syva MicroTrak Test. J Clin Microbiol. 1988 Mar;26(3):493–497. doi: 10.1128/jcm.26.3.493-497.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Dolter J., Bryant L., Janda J. M. Evaluation of five rapid systems for the identification of Neisseria gonorrhoeae. Diagn Microbiol Infect Dis. 1990 May-Jun;13(3):265–267. doi: 10.1016/0732-8893(90)90070-c. [DOI] [PubMed] [Google Scholar]
  9. Gill M. J. Serotyping Neisseria gonorrhoeae: a report of the Fourth International Workshop. Genitourin Med. 1991 Feb;67(1):53–57. doi: 10.1136/sti.67.1.53. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Granato P. A., Franz M. R. Use of the Gen-Probe PACE system for the detection of Neisseria gonorrhoeae in urogenital samples. Diagn Microbiol Infect Dis. 1990 May-Jun;13(3):217–221. doi: 10.1016/0732-8893(90)90062-z. [DOI] [PubMed] [Google Scholar]
  11. Healey K., Chandler H. M., Cox J. C., Hurrell J. G. A rapid semi quantitative capillary enzyme immunoassay for digoxin. Clin Chim Acta. 1983 Oct 31;134(1-2):51–58. doi: 10.1016/0009-8981(83)90183-3. [DOI] [PubMed] [Google Scholar]
  12. Hossain A., Bakir T. M., Siddiqui M., De Silva S. Enzyme immunoassay (EIA) in the rapid diagnosis of gonorrhoea. J Hyg Epidemiol Microbiol Immunol. 1988;32(4):425–431. [PubMed] [Google Scholar]
  13. Ison C. A., Tanna A., Easmon C. S. Evaluation of a fluorescent monoclonal antibody reagent for identification of cultured Neisseria gonorrhoeae. J Med Microbiol. 1988 Jun;26(2):121–123. doi: 10.1099/00222615-26-2-121. [DOI] [PubMed] [Google Scholar]
  14. Knapp J. S., Holmes K. K., Bonin P., Hook E. W., 3rd Epidemiology of gonorrhea: distribution and temporal changes in auxotype/serovar classes of Neisseria gonorrhoeae. Sex Transm Dis. 1987 Jan-Mar;14(1):26–32. [PubMed] [Google Scholar]
  15. Kuritza A. P., Edberg S. C., Chapis C., Gallo P. Identification of Neisseria gonorrhoeae with the ORTHOProbe DNA probe test. Diagn Microbiol Infect Dis. 1989 Mar-Apr;12(2):129–132. doi: 10.1016/0732-8893(89)90002-3. [DOI] [PubMed] [Google Scholar]
  16. Lewis J. S., Kranig-Brown D., Trainor D. A. DNA probe confirmatory test for Neisseria gonorrhoeae. J Clin Microbiol. 1990 Oct;28(10):2349–2350. doi: 10.1128/jcm.28.10.2349-2350.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Lind I. Epidemiology of antibiotic resistant Neisseria gonorrhoeae in industrialized and developing countries. Scand J Infect Dis Suppl. 1990;69:77–82. [PubMed] [Google Scholar]
  18. Lussier M., Brodeur B. R., Winston S. Detection of Neisseria gonorrhoeae by dot-enzyme immunoassay using monoclonal antibodies. J Immunoassay. 1989;10(4):373–394. doi: 10.1080/01971528908053248. [DOI] [PubMed] [Google Scholar]
  19. Panke E. S., Yang L. I., Leist P. A., Magevney P., Fry R. J., Lee R. F. Comparison of Gen-Probe DNA probe test and culture for the detection of Neisseria gonorrhoeae in endocervical specimens. J Clin Microbiol. 1991 May;29(5):883–888. doi: 10.1128/jcm.29.5.883-888.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Papasian C. J., Bartholomew W. R., Amsterdam D. Validity of an enzyme immunoassay for detection of Neisseria gonorrhoeae antigens. J Clin Microbiol. 1984 Mar;19(3):347–350. doi: 10.1128/jcm.19.3.347-350.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Rossau R., Duhamel M., Van Dyck E., Piot P., Van Heuverswyn H. Evaluation of an rRNA-derived oligonucleotide probe for culture confirmation of Neisseria gonorrhoeae. J Clin Microbiol. 1990 May;28(5):944–948. doi: 10.1128/jcm.28.5.944-948.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Vera H. D. A Simple Medium for Identification and Maintenance of the Gonococcus and Other Bacteria. J Bacteriol. 1948 Apr;55(4):531–536. doi: 10.1128/jb.55.4.531-536.1948. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Yong D. C., Prytula A. Rapid micro-carbohydrate test for confirmation of Neisseria gonorrhoeae. J Clin Microbiol. 1978 Dec;8(6):643–647. doi: 10.1128/jcm.8.6.643-647.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Clinical Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES