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. Author manuscript; available in PMC: 2010 Feb 24.
Published in final edited form as: Biochemistry. 2009 Feb 24;48(7):1454–1461. doi: 10.1021/bi801874n

Figure 4. Coregulator binding patterns and specificity determinants.

Figure 4

The equilibrium binding constants (Kd values) for the binding of VDR to coregulator peptides (SRC1, SRC2, SRC3, DRIP205, P300, RIP140-5, NCoR, SMRT and Hr) in the presence and absence of 1α,25(OH)2D3 or LG190178 were determined by fluorescent polarization. IC50 values of peptides determined by a competition pull down assay employing VDR-LBD and full length SRC2 in the presence of 1α,25(OH)2D3. The Kd and IC50 values are color coded: dark green (<3 μM), green (3.1-20.0 μM), light green (>20 μM), grey (>50 μM, weak or no binding).