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. 2009 Mar 27;5(3):e1000356. doi: 10.1371/journal.ppat.1000356

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Dickerson et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) Reporter constructs containing no promoter (pGL3-basic), the R promoter (Rp-LUC) or the Na promoter (Nap-LUC) driving luciferase were transfected into HONE-1, HaCaT or DG75 cells. The luciferase activity produced by each vector is shown (relative to the luciferase activity of the promoterless vector, set as 1). HeLa (B) and DG75 (C) cells were transfected with reporter constructs containing no promoter (none) or the Na promoter (Nap) driving luciferase. Luciferase vectors were either mock-methylated or methylated in vitro using M.Sss1 prior to transfection as indicted. The decrease in luciferase activity derived from the methylated versus unmethylated promoter constructs is indicated (activity of the unmethylated constructs is set at 100%).