Skip to main content
. 2009 Mar 27;5(3):e1000356. doi: 10.1371/journal.ppat.1000356

Figure 4. Z preferentially binds to the methylated forms of ZRE sites in the Nap.

Figure 4

(A) The ability of in vitro translated Z to bind to labeled oligonucleotide probes containing potential ZRE sites in the Nap was examined by EMSA using methylated or mock-methylated probes as indicated. Nap sequences (with probable ZRE sites underlined) were (Nap ZRE1, −58 to −41): 5′-CAT TCT CGC CCG TGG GCC-3′ and (Nap ZRE2, −83 to −70): 5′-GTT GAG CGT GGC CA-3′. Binding to the unmethylated and methylated forms of two CpG-containing ZREs in the BRLF1 promoter (Rp) was also examined. The relative efficiency of Z binding to each probe is indicated; the amount of Z binding to the methylated Rp ZRE3 probe is arbitrarily set as 100%. ND: none detected. (B) The ability of in vitro translated Z to bind to the labeled oligonucleotide Nap ZRE1 probe (−58 to −41) containing no methylated cytosine, one methylated cytosine, or two methylated cytosines was compared. The methylated cytosine(s) present in each probe are indicated by a bold C in the sequence.