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. 2009 Mar 27;5(3):e1000356. doi: 10.1371/journal.ppat.1000356

Figure 6. Serine residue 186 is required for Z binding to the ZREs in the Nap.

Figure 6

(A) EMSA was performed using either methylated or mock-methylated oligonucleotide probes containing the Nap ZRE1, Nap ZRE2, Rp ZRE2, Rp ZRE3 and BMRF1p AP1 sites and in vitro translated wild-type Z (WTZ), ZS186A or ZC189S. The relative efficiency of WTZ, versus mutant Z, binding to each probe is indicated; the amount of WTZ binding to each probe is arbitrarily set as 100%. ND: none detected. (B) The amount of Z protein used in the EMSAs in (a) was quantitated by immunoblot for WT and mutant proteins.