Figure 2.

The effect of SiRNA-mediated inhibition of β-catenin on myogenic differentiation. A, Random SiRNA and β-catenin SiRNA (SiRNA1 and SiRNA2) transfected cells were treated with or without 100 nm testosterone (T, 100 nm) for 12 d, and the images of cells were obtained using a bright-field microscope. B, Cells were transfected with 100 nm either random SiRNA (Ran SiRNA) or β-catenin-specific SiRNA (SiRNA1 and SiRNA2) for 16 h and subsequently maintained in regular growth medium for 56 h for Western blot analysis of β-catenin levels. The experiment was repeated three times. C, Cells were transfected either with Ran SiRNA or β-catenin-specific SiRNA1 or SiRNA2 as described in B and treated with 100 nm testosterone (T). Cells were analyzed for MHC II (after 12 d) and MyoD (after 3 d) protein levels by Western blot. Three independent experiments were performed, and representative data are shown.