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. 2008 Nov 6;150(3):1485–1494. doi: 10.1210/en.2008-1459

Figure 6.

Figure 6

Hormonal regulation of D1. Panel A, Dual-immunofluorescent detection of PRA and D1 was determined for ovariectomized adult BALB/c and C57BL/6 mice after 10 d control (C), P, or E plus P. Nuclear expression of D1 was detected (green nuclei); nuclei (blue) were counterstained with DAPI. Scale bar, 25 μm. Panel B, Quantitation of D1+ PRA− and PRA+D1+ luminal epithelial cell nuclei. *, Total nuclear D1+ cells after 10 d P and E plus P is greater than in 10 d control in BALB/c glands (P < 0.05); #, total nuclear D1+ cells after 10 d E plus P is greater than after 10 d control or P in C57BL/6 glands (P < 0.05). The values represent the mean ± sem. Panel C, Whole mounts from virgin adult and 1 d postpartum (pp) D1−/− and wild-type (WT) BALB/c mice.