Figure 2.

Effects of elevated SF-1 on aldosterone and cortisol production. H295R/TR/SF-1 cells were incubated with doxycycline (Doxy; 0.25 μg/ml) for 72 h. Cells were then treated with or without Ang II (10 nm) for 24 h. Aldosterone (A) and cortisol (B) levels were then measured in the experimental medium. Values represent the mean ± se from three independent experiments each ran in triplicate. *, P < 0.01.