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SF-1 effects on steroidogenic enzyme mRNA expression. H295R/TR/SF-1 cells were treated with doxycycline (Doxy; 0.25 μg/ml) for 72 h followed by treatment with or without Ang II (10 nm) for 24 h. Real-time quantitative RT-PCR was used to measure CYP11B1 (A), CYP11B2 (A), CYP21 (A), CYP11A1 (B), HSD3B2 (B), and StAR (B) mRNA expression. Values represent the mean ± se from three independent experiments each run in triplicate. *, P < 0.05; **, P < 0.01.
