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. 2008 Oct 30;150(3):1303–1309. doi: 10.1210/en.2008-0667

Figure 5.

Figure 5

Effects of SF-1 siRNA on SF-1, CYP11B1, and CYP11B2 expression. Both endogenous and transgene SF-1 expression were silenced in H295R/TR/SF-1 cells by RNA interference using Nucleofector technology. Cells were transfected with 10 nm SF-1-specific Stealth siRNA or with a control siRNA oligo (scrambled oligo) and allowed to recover for 48 h. During the final 24 h, cells were treated with or without doxycycline (0.25 μg/ml) and then harvested for total RNA isolation and real-time quantitative RT-PCR. Data points represent the mean ± se from three independent experiment. *, P < 0.01.