Table 1.
Sequences of primers and probes used for quantification of gene expression by real-time RT-PCR
| Gene | Sequence (5′–3′) |
|---|---|
| StAR | |
| Forward primer | ATGAGTAAAGTGGTCCCAGATG |
| Reverse primer | ACCTTGATCTCCTTGACATTGG |
| Probe | ATCCGGCTGGAGGTCGTGGTGGACGATC |
| CYP11A1 | |
| Forward primer | GAGATGGCACGCAACCTGAAG |
| Reverse primer | CTTAGTGTCTCCTTGATGCTGGC |
| Probe | CGATCTGCCGCGCAGCCAAGACCTCTGAT |
| HSD3B2 | |
| Forward primer | GCGGCTAATGGGTGGAATCTA |
| Reverse primer | CATTGTTGTTCAGGGCCTCAT |
| Probe | TGATACCTTGGTACACTTGTGCGTTAAGACCCA |
| CYP21 | |
| Forward primer | ACCTCAGTTTCTCCTTTATTGC |
| Reverse primer | AGAGCCAGGGTCCTTCAC |
| Probe | CGCGATCAGGAAGCCTTCTCTGCCAGCGAGATC |
| CYP11B1 | |
| Forward primer | GGCAGAGGCAGAGATGCTG |
| Reverse primer | TCTTGGGTTAGTGTCTCCACCTG |
| Probe | TGCTGCACCATGTGCTGAAACACCT |
| CYP11B2 | |
| Forward primer | GGCAGAGGCAGAGATGCTG |
| Reverse primer | CTTGAGTTAGTGTCTCCACCAGGA |
| Probe | CTGCACCACGTGCTGAAGCACT |