Figure 2.

Distribution of D. rerio proSAAS. proSAAS gene expression was observed in neural tissues, which are telencephalon (te), diencephalon (dc), midbrain (mb), hindbrain (hb), spinal cord (sc), pituitary (arrows in C and E), and cranial nerves (arrowheads in D and F) at 1 dpf (A, B, and I), 2 dpf (C and D), 3 dpf (E and F), and 5 dpf (G and H). Observations made at high magnification around the left optic capsule revealed the proSAAS expression in cranial nerves of gIX and gX (arrowheads in J). In nonneural tissue the proSAAS gene was expressed in pancreatic cells and its primordium, shown as asterisks (A, C–F, and I) and superimposed in D and F. Samples hybridized with sense probe did not show any staining (K). The orientation is anterior at the left in all figures. A, C–E, G, and I–K are from a lateral view. B, D, and F are from a dorsal view, and H is from a ventral view. e, Eye; ht, hypothalamus; nc, notochord; oc, otic capsule; t, tegmentum; th, thalamus.