Table 1.
External and internal primer sequences of Trks and p75NTR for Amplification
| Primer | Primer sequence | GenBankAccession No. | Nucleotide Position (bp) |
|---|---|---|---|
| External | |||
| TrkA | F: 5’GTGGAGAAGAAGGACGAA ACAC | NM_002529 | 1222-1243 |
| R: 5’GTATTGTGGGTTCTCGATGATG | 1467-1488 | ||
| TrkC | F: 5’TACAAGCTTTAACCGGCTCACCACACT CTC | S_76475 | 408-428 |
| R: 5’TACGAATTCCCACCACGT TCTCTGCAA TGC | 851-871 | ||
| p75NTR | F: 5’CTGCAAGCAGAACAAGCAAGGAGC | NM_002507 | 831-854 |
| R: 5’AGGCCTCATGGGTAAAGGAGT | 1121-1141 | ||
| NSE | F: 5’GGGACTGAGAA CAAATCCAAG | NM_001975 | 295-315 |
| R: 5’CTCCAAGGCTTCACTGTTCTC | 655-675 | ||
| Cyclophilin | F: 5’AGCACTGGAGAGA AAGGATTTG | XM_371409 | 118-139 |
| R: 5’CCTCCACAAT ATTCATGCCTTC | 400-421 | ||
| Internal | |||
| TrkA | F: 5’TGGGATCAACCTCGAGGCTGTGC TGG | 1344-1369 | |
| TrkC | F: 5’GTGTGACCTTCTCGAGATCAGCGTG | 618-642 | |
| p75NTR | F: 5’ACGCAGACAGCCTCGAGCCAGGCCCT | 961-990 | |
| NSE | 5’GGCAACAAGCTC GAGATGCAGGAGTTC | 478-504 | |
| Cyclophilin | F:5’GGTGGCAAGTCCATCTAT/AAATGCTGGACCCAACAC | 220-237/303-320 |
F, forward; R, reverse; bold and italicized letters indicate the mutated bases. Underline bases indicate the Xho I cleavage site.