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. Author manuscript; available in PMC: 2009 Mar 12.
Published in final edited form as: J Am Chem Soc. 2008 Apr 9;130(17):5667–5669. doi: 10.1021/ja711244h

Figure 2.

Figure 2

Semisynthetic PKA. (A) Route to semisynthetic PKA linked to ATP (PKAATP) or phosphate (PKAP) at the C-terminal phosphorylation site Ser338. Natural (top) and semisynthetic (bottom) PKA sequences from 301 to 351 with synthetic peptide region underlined and Ser338 has an asterisk. (B) SDSPAGE and Western blot analysis of semisynthetic PKAs compared to commercially available [PKA(com)] and full length recombinant PKA [PKA(recomb)]. Western blot with anti-phosphoThr197 (T500P) showed comparable activation loop phosphorylation.