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A model system for assaying RecZF-targeted integration. Donor plasmid and RecZF expression vector were cotransfected into a human cell line containing a single genomic target site (naïve, N). Bidirectional integration yielded 2 phenotypically distinct products. In one case, the CMV promoter lies adjacent to EGFP, enhancing cellular fluorescence (EGFP-high). In the other, CMV promoter and EGFP are distant and in opposite orientations (EGFP-low).
