Table 2.
Analysis of transient carbohydrates in control and complemented lines
Representative line | Carbohydrate | Lamina | petiole |
WT | Glu | 2.28 ± 0.95 | 0.51 ± 0.02 |
(Col-0)a | Fru | 0.80 ± 0.36 | 0.14 ± 0.03 |
Suc | 1.72 ± 0.76 | 0.35 ± 0.07 | |
Starchb | 59.53 ± 4.96 | 8.28 ± 3.68 | |
Total | 64.33 ± 4.42 | 9.28 ± 3.79 | |
SUC2p::cSUC2 | Glu | 1.40 ± 0.42 | 0.53 ± 0.15 |
(line 1039)a | Fru | 0.42 ± 0.12 | 0.12 ± 0.02 |
Suc | 1.40 ± 0.40 | 0.35 ± 0.07 | |
Starch | 84.60 ± 18.20c | 12.02 ± 3.64 | |
Total | 87.83 ± 18.17c | 13.02 ± 3.60 | |
CoYMVp::cSUC2 | Glu | 1.11 ± 0.22 | 0.55 ± 0.08 |
(line 1070) | Fru | 0.41 ± 0.13 | 0.14 ± 0.03 |
Suc | 1.90 ± 0.07 | 0.43 ± 0.04 | |
Starch | 46.55 ± 4.50d | 10.92 ± 2.55 | |
Total | 49.96 ± 4.20d | 12.04 ± 2.56 | |
RolCp::cSUC2 | Glu | 15.41 ± 4.24c | 0.67 ± 0.15 |
(line 1133) | Fru | 3.44 ± 1.05c | 0.17 ± 0.04 |
Suc | 11.50 ± 1.79c | 0.52 ± 0.03c | |
Starch | 547.63 ± 117.90c | 34.50 ± 6.80c | |
Total | 577.97 ± 113.92c | 35.87 ± 6.98c |
Sugar and starch in the lamina (excluding midrib) and petiole of the indicated plant lines after 30 days of growth. The first three mature leaves from each plant were pooled for extraction, and variation (standard deviation) calculated from n = 3 to 5 sibling plants. Sugars levels are expressed as nmoles per milligram fresh weight, and starch is expressed as glucose equivalents.
a [25]
b Expressed as glucose equivalents
c Significant increase relative to wild type; Students T-test, p < 0.05
d Significant decrease relative to wild type; Students T-test, p < 0.05