Table 1.
Development of mouse 2-cell embryos after exposure to visible light for 30 min followed by co-culture with either MEF or HEF
| Experimental groups | No. of embryos | Development (%) | ||
|---|---|---|---|---|
| Day 3 | Day 4 | Day 5 | ||
| MEM0 | 40 | 24 (60) | 19 (48) | 21(52) |
| MEM30 | 40 | 23 (57) | 9 (22) | 8 (20) |
| HEF0 | 40 | 32 (80) | 30 (75) | 33 (82) |
| HEF30 | 42 | 28 (67) | 28 (67) | 28 (67)** |
| MEF0 | 40 | 35 (87) | 32 (80) | 32 (80) |
| MEF30 | 43 | 29 (67) | 25 (58) | 25 (58)* |
Experiments were replicated eight times. MEM0 is un-exposed embryos cultured in MEM- α; MEM30 is the groups of embryos exposed to visible light for 30 min followed by culture in MEM- α; HEF0 and MEF0 are un-exposed embryos co-cultured with HEF and MEF respectively; HEF30 and MEF30 are the groups of embryos exposed to visible light for 30 min followed by co-culture with HEF and MEF respectively. Day 3, 4 and 5 are days in which embryos developed beyond morula, expanded blastocyst and hatching or hatched blastocyst stage respectively
*P < 0.05 and **P < 0.01 Comparing to MEM30