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. 2009 Mar 27;5(3):e1000440. doi: 10.1371/journal.pgen.1000440

Figure 2. MYB21, MYB24I, and MYB57 Are RGA- and RGL2-Repressible Floral Specific Genes.

Figure 2

(A) RT-PCR analysis shows that the expression of the three MYB genes in the young flower buds are greatly reduced in ga1-3 but restored to the WT level in ga1-3 gai-t6 rgat2 rgl1-1 rgl2-1 (penta). (B–C) RT-PCR analysis shows that the repressed expression of the three MYB genes in ga1-3 was restored in ga1-3 gai-t6 rga-t2 rgl2-1 (Q2) and ga1-3 rga-t2 rgl1-1 rgl2-1 (Q4) two quadruple mutants but not in ga1-3 gai-t6 rgl1-1 rgl2-1 (Q1) and ga1-3 gai-t6 rga-t2 rgl1-1 (Q3) two quadruple mutants (B). This restoration of MYB expression nicely correlates with the recovery of fertility in Q2 and Q4 (C). Total RNA used in RT-PCR analysis was extracted from the young flower buds. (D) RT-PCR analysis shows that the three MYB genes are floral specific genes. IF, inflorescence; CL, cauline leaves; RL, rosette leaves; IT, internodes; RT, roots; SL, siliques. (E) Amino acid alignment of MYB21, MYB24 and MYB57 proteins. The conserved R2 and R3 domains and the NYWSV/ME/DDlWP/S motif are highlighted in red, blue and green, respectively.