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. 2009 Apr 3;5(4):e1000442. doi: 10.1371/journal.pgen.1000442

Figure 2. High temporal resolution profiling of NIH3T3 and U2OS cells reveals severely dampened circadian output.

Figure 2

NIH3T3 and U2OS cells were grown to confluence and shocked with either forskolin (NIH3T3) or dexamethasone (U2OS) to synchronize their circadian clocks. mRNA samples were collected every h for 48 h and profiled on Affymetrix expression arrays. Rhythmic genes were identified using both COSOPT and Fisher's G-test at a false-discovery rate of <0.05. The period length of every rhythmic transcript was plotted as a histogram (A–B). To demonstrate that core clock genes cycle well in these data sets, panels C–F show the microarray intensity from two representative genes was plotted against CT time for both NIH3T3 and U2OS cells. NR1D2 (C–D) and Per3 (E–F) expression profiles show examples of cycling 24 h genes.