Table 3.
Serum IL-1β, IL-6, and TNF Levels (pg/mL) at Different Times Over a 24-Hour Period in Control Conditions and After a 3-Day Administration of 0.25 mg Melatonin/kg of Body Weight (b.w.) and 2.5 mg Melatonin/kg of b.w. to Young and Old Ringdoves, respectively, 1 Hour Before Lights Off (7 PM) (A), or 300 mg Tryptophan/kg of b.w. to Old Ringdoves 1 Hour After Lights on (9 AM) (B)
| Young Animals (Mel 0.25 mg/kg of b.w.) | Old Animals (Mel 2.5 mg/kg of b.w.) | Old animals (Trp 300 mg/kg of b.w.) | |||||||||||||||||
| IL-1β | IL-6 | TNF | IL-1β | IL-6 | TNF | IL-1β | IL-6 | TNF | |||||||||||
| A | Control | Treated | Control | Treated | Control | Treated | Control | Treated | Control | Treated | Control | Treated | B | Control | Treated | Control | Treated | Control | Treated |
| 9:00 PM | — | — | 2.3 ± 1.0 | 4.4 ± 1.0 | — | — | 54.4 ± 5.4* | 33.0 ± 5.4† | 3.3 ± 1.0 | 6.5 ± 1.0 | 14.3 ± 1.0* | 10.5 ± 1.0† | 10:00 AM | — | — | 1.7 ± 1.5 | 3.0 ± 1.0 | 11.0 ± 1.0* | — |
| 10 PM | 8.9 ± 2.5 | — | 2.8 ± 1.0 | 5.8 ± 1.0† | — | — | 42.1 ± 2.1* | 43.0 ± 2.1 | 1.2 ± 1.5 | 5.5 ± 2.5 | 12.5 ± 1.5* | — | Noon | 12.5 ± 2.5* | 6.3 ± 3.5 | 3.8 ± 1.0 | 3.0 ± 1.0 | — | — |
| 11:00 PM | — | — | 5.2 ± 2.0 | 3.0 ± 0.5† | — | — | 30.3 ± 5.4* | 14.4 ± 5.4* | 1.2 ± 1.5 | 4.0 ± 1.5 | 10.9 ± 1.5* | — | 3:00 PM | — | — | 6.5 ± 2.0† | 4.4 ± 2.0 | 5.5 ± 0.5* | — |
| Midnight | 9.4 ± 3.6 | 4.5 ± 1.5 | 4.6 ± 1.5 | 3.0 ± 0.5† | — | — | 18.5 ± 4.5* | 13.0 ± 4.5 | 0.7 ± 1.5 | 3.4 ± 2.0 | 2.0 ± 0.2* | — | 6:00 PM | 1.6 ± 0.5* | 1.6 ± 0.5 | 2.3 ± 0.5 | 7.9 ± 4.0 | 4.3 ± 2.0* | — |
| 1:00 AM | — | — | 5.7 ± 1.0 | 3.4 ± 0.5† | — | — | — | — | 2.3 ± 2.0 | 3.6 ± 1.0 | 6.5 ± 1.0* | 1.75 ± 0.5† | 9:00 PM | 54.4 ± 5.4* | — | 3.3 ± 1.0 | 4.1 ± 1.0 | 14.3 ± 1.0* | 7.5 ± 1.0* |
| 2:00 AM | — | — | 5.7 ± 2.0 | 1.6 ± 0.5† | — | — | — | — | 2.3 ± 1.5 | 0.2 ± 1.0 | 5.3 ± 1.0* | — | 10:00 PM | 42.1 ± 2.1* | 10.3 ± 2.1† | 1.2 ± 1.5 | 5.6 ± 2.5 | 12.5 ± 1.5* | 9.0 ± 0.5* |
| 3:00 AM | 18.9 ± 4.5 | — | 6.2 ± 1.0 | 3.0 ± 1.0† | — | — | — | — | 3.3 ± 2.0 | 4.8 ± 1.0 | 7.5 ± 1.0* | — | 11:00 PM | 30.3 ± 5.4* | — | 1.2 ± 1.5 | 3.7 ± 0.5 | 10.9 ± 1.5* | — |
| 4:00 AM | — | — | 6.5 ± 1.0 | 5.4 ± 1.0 | — | — | 12.6 ± 2.5* | — | 3.1 ± 2.0 | 3.4 ± 1.0 | — | 4.3 ± 1.0† | Midnight | 18.5 ± 4.5* | — | 0.7 ± 1.5 | 2.7 ± 2.0 | 2.0 ± 0.2* | — |
| 5:00 AM | — | — | 2.8 ± 1.0 | 5.1 ± 1.0† | — | — | 33.5 ± 3.4* | 10.3 ± 3.4† | 3.3 ± 1.0 | 5.1 ± 1.0 | — | — | 1:00 AM | — | — | 2.3 ± 2.0 | 3.7 ± 1.0 | 6.5 ± 1.0* | — |
| 6:00 AM | 63.9 ± 3.9 | — | 5.2 ± 1.0 | 2.3 ± 1.0† | — | — | 81.2 ± 6.8* | 31.2 ± 6.8† | 3.6 ± 1.0 | 4.1 ± 1.0 | — | — | 2:00 AM | — | — | 2.3 ± 1.5 | 3.2 ± 1.0 | 5.3 ± 1.0* | — |
| 9:00 AM | — | — | 4.1 ± 2.0 | 5.8 ± 2.0 | — | — | — | — | 1.7 ± 1.5 | 5.1 ± 2.0 | 11.0 ± 1.0* | 9.3 ± 1.0 | 3:00 AM | — | — | 3.3 ± 2.0 | 3.4 ± 1.0 | 7.5 ± 1.0* | — |
| Noon | — | — | 3.1 ± 2.0 | 5.0 ± 2.0 | — | — | 12.5 ± 2.5* | 10.6 ± 2.5 | 3.8 ± 1.0 | 2.0 ± 1.0 | — | — | 4:00 AM | 12.6 ± 2.5* | — | 3.1 ± 2.0 | 3.0 ± 1.0 | — | — |
| 3:00 PM | — | 3.5 ± 0.5 | 2.3 ± 1.5 | 4.9 ± 1.5 | — | — | — | — | 6.5 ± 2.0* | 7.9 ± 2.0 | 5.5 ± 0.5* | 3.3 ± 1.0† | 5:00 AM | 33.5 ± 3.4* | — | 3.3 ± 1.0 | 5.4 ± 1.0 | — | — |
| 6:00 PM | 19.8 ± 6.8 | 3.4 ± 0.4† | 3.3 ± 1.5 | 4.1 ± 1.5 | — | — | 1.6 ± 0.5* | — | 2.3 ± 0.5 | 5.8 ± 2.0 | 4.3 ± 2.0* | — | 6:00 AM | 81.2 ± 6.8* | — | 3.6 ± 1.0 | 5.6 ± 1.0 | — | — |
Notes: Each value represents the mean ± SD of 10 determinations performed in duplicate. IL = interleukin; TNF = tumor necrosis factor.
*
p < .01 with respect to their respective values in the young animals. (—) Undetected values.
†
p < .01 with respect to the values obtained in their respective control groups.