. 2009 Jan 30;64A(3):325–331. doi: 10.1093/gerona/gln052

Table 1.

Primers Used in Real-Time PCR

Primer	Sequence
iNOS	Forward: TGAGAGAGCAGAGGAAAATGAAC
iNOS	Reverse: CAAGGAATTATACACGGAAGGG
TNF-α	Forward: CCTTATCTACTCCCAGGTTCTC
TNF-α	Reverse: TTTCTCCTGGTATGAAATGGC
18s rRNA	Forward: TCAAGAACGAAAGTCGGAGGTT
18s rRNA	Reverse: GGACATCTAAGGGCATCACAG
GAPDH	Forward: TCCACCACCCTGTGCTGTA
GAPDH	Reverse: ACCACAGTCCATGCCATCAC

Note: iNOS, inducible nitric oxide synthase; TNF-α, tumor necrosis factor alpha. Used for the real-time polymerase chain reaction experiments. glyceraldehyde 3-phosphate dehydrogenase (GAPDH) was used as a housekeeping gene for the retina and 18s ribosomal RNA (rRNA) for the choroid, because we have observed changes in GAPDH with age in the choroid.