Fig. 4.

Dimerization of PrgX is restored upon addition of excess iCF10. OG1RF/p043lacZ cells were treated with 1% formaldehyde, lysed, and the resulting lysates were analysed by Western blotting for PrgX. During the growth of cells used for protein extraction, cCF10 was added at 25 ng ml⁻¹ and excess quantities of iCF10 (5X = 125 ng ml⁻¹; 10X = 250 ng ml⁻¹; 50X = 1.25 μg ml⁻¹; 100X = 2.5 μg ml⁻¹) were added to aliquots of this culture immediately following pheromone addition. Prior to cell lysis, each culture was split, and one portion was treated with formaldehyde to cross-link PrgX, whereas the other was not treated with formaldehyde.