Table 1.
RT-PCR Ratios
| Gene | Rv | Condition | 5 weeks | 10 weeks | 15 weeks | 15 weeks steroids | 20 weeks steroids |
|---|---|---|---|---|---|---|---|
| Hypothetical Protein (DNA repair) | Rv2191 | Macrophages | 0.063 ± 0.040 (3) | 0.005 ± 0.003 (3) | 1.665 ± 0.996 (6) P=0.05a | 0.959 ± 0.810 (6) P=0.012a | 0.313 ± 0.208 (6) P=0.05a |
| aceAa (isocitrate lyase) | Rv1915 | Macrophages | 0.012 ± 0.006 (4) | 0.009 ± 0.005 (4) | 0.003 ± 0.002 (2) | 0.007 ± 0.006 (3) | 0.005 ± 0.004 (2) |
| fbpA (Antigen 85A) | Rv3804c | Macrophages | - | 0.0006 ± 0.0015 (1) | - | 0.0044 ± 0.0090 (3) | 0.0005 ± 0.0013 (1) |
| Hypothetical Protein | Rv1735c | Macrophages | - | 0.0009 ± 0.0014 (3) | - | - | - |
| Conserved Hypothetical Protein | Rv2050 | Starvation | 0.0002±0.0001 (2) | 0.008 ± 0.006 (2) | 0.010 ± 0.005 (4) | 0.021 ± 0.018 (4) | |
| Conserved Hypothetical Protein | Rv3131 | Starvation, acid | 0.0002 ± 0.0002 (1) | 0.0006 ± 0.0003 (3) | 0.008 ± 0.007 (3) | ||
| lat | Rv3290c | Starvation | 0.0002 ± 0.0002 (1) | 0.004 ± 0.002 (3) | 0.0001 ± 0.0001 (1) | 0.0009 ± 0.0009 (1) | |
| Hypothetical Protein | Rv2660c | Starvation | 13.00 ± 31.84 (1) | 5.54 ± 13.57 (1) | - | - | 0.77 ± 1.87 (1) |
| Hypothetical Protein | Rv0188 | Starvation | 0.0004 ± 0.0009 (1) | 0.0045 ± 0.0109 (1) | - | - | - |
| Conserved Hypothetical Protein | Rv2626c | Hypoxia | 0.085 ± 0.051 (3) | 0.077 ± 0.039 (3) | 0.944 ± 0.663 (2) | 2.304 ± 3.066 (2) | 2.892 ± 2.865 (2) |
| Hypothetical Protein (transcriptional protein) | Rv0792c | Hypoxia | 0.017 ± 0.009 (4) | 0.006 ± 0.003 (2) | 0.001 ± 0.001 (1) | 0.002 ± 0.002 (1) | |
| fdxA | Rv2007c | Hypoxia | - | 0.0006 ± 0.0015 (1) | 0.0044 ± 0.0090 (3) | 0.0005 ± 0.0013 (1) | |
| icl1 (isocitrate lyase) | Rv0467 | Hypoxia | 0.0005 ± 0.0013 (2) | - | - | - | - |
| rpfB (resuscitation promoting factor) | Rv1009 | Resuscitation | 0.018 ± 0.011 (3) | 0.539 ± 0.389 (4) | 0.075 ± 0.048 (2) | 0.268 ± 0.173 (3) | 0.028 ± 0.015 (3) |
| rpfC (resuscitation promoting factor) | Rv1884c | Resuscitation | 0.048 ± 0.025 (4) | 0.139 ± 0.137 (2) | 0.089 ± 0.091 (2) | 0.306 ± 0.202 (4) | |
| Conserved Hypothetical | Rv3402c | Iron-induced | 0.067 ± 0.054 (4) | 0.005 ± 0.002 (3) | 0.009 ± 0.007 (2) | 0.056 ± 0.047 (3) | 0.005 ± 0.003 (3) |
| Conserved Hypothetical | Rv3615c | Acid | 0.0010 ± 0.0024 (1) | 0.0049 ± 0.0078 (3) | - | - | 0.258 ± 0.559 (3) |
| Monoxygenase | Rv3083 | Acid | 0.043 ± 0.106 (1) | - | - | - | - |
| Conserved Hypothetical Protein (DNA repair) | Rv2816c | Other | 0.283 ± 0.243 (4) | 0.226 ± 0.128 (3) | 1.956 ± 0.984 (4) P=0.181 | 1.082 ± 0.717 (4) | 34.769 ± 33.182 (3) |
a
P-value compared to 5 week value
Numbers denote the number of samples in which a particular gene transcript was detected by RT-PCR is listed in parentheses. 16S rRNA transcripts were detected for all 6 samples at every time point listed in table.