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. Author manuscript; available in PMC: 2009 Dec 9.
Published in final edited form as: Biochemistry. 2008 Dec 9;47(49):13056–13063. doi: 10.1021/bi8015197

Figure 2.

Figure 2

Reactions of carbachol with AChE monitored by thioflavin T fluorescence. Reactions were initiated by stopped-flow mixing of AChE with carbachol and thioflavin T as outlined in the Experimental Procedures, and the fluorescence F was recorded continuously. Initial concentrations of thioflavin T (15.6 μM) and AChE (152 nM) were the same in each reaction, while the respective concentrations of carbachol were 0, 0.5, 10, 40, and 60 mM (from the upper to the lower trace taken at time t = 0). FB (eq 5) from the components individually was 83.