Figure 1.

TP53 gene sequencing results from laser capture microdissected (LCM) samples. Top row showed representative images of H&E staining of resting endometrium (RE), endometrial glandular dysplasia (EmGD), and ESC with an apparently non-invasive growth pattern (EIC/ESC) and p53 immunohistochemical staining of morphologically normal endometrial cells (p53 signatures). (original magnifications: 200 ×). The degree of nuclear atypia in EmGD (the glands with *) clearly exceeds that of the RE but falls short of EIC/ESC. Samples of p53 signatures, EmGD, and EIC/ESC from DNA sequence analyses showed identical p53 gene mutations of exon 7 at codon 248 from CGG to TGG (Arg to Trp), while no mutation was found in the corresponding RE sample. Identical mutation was also observed in area of invasive ESC (not shown) in the same uterus. These samples were obtained from one of the cases previously studied in Zheng laboratory.