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. Author manuscript; available in PMC: 2009 Aug 14.
Published in final edited form as: Neuron. 2008 Aug 14;59(3):399–412. doi: 10.1016/j.neuron.2008.06.023

Figure 1. TrkB is expressed by hippocampal NPCs.

Figure 1

(A) In situ hybridization analysis of TrkB and BDNF mRNA in the adult dentate gyrus (DG). In the high magnification image (right), note the distribution of silver gram (black spheres) in all cells. Scale bars, 1mm (low-magnification) and 10um (high-magnification). GL, granular layer; SGZ, sub-granular zone.

(B) Confocal image of the DG of an adult Nestin-GFP transgenic mouse, co-immunostained for GFP (green), NeuroD (red) and NeuN (blue). GFP expression was restricted to NPCs and did not colocalize with immature (NeuroD+) or mature (NeuN+) neurons. Insert showed a DG derived neurosphere that expresses GFP. Scale bars, 10um.

(C) RT-PCR detection of TrkB and BDNF transcripts in FACS sorted Nestin-GFP positive cells and DG derived neurospheres. NS, neurosphere.

(D) Immuno-staining for TrkB (green) on adult DG sections from wild-type and TrkBhGFAP mice (left panels). Co-staining for TrkB (green) and Ki67 (red), or Doublecortin (red) demonstrated co-localization of TrkB with proliferating (Ki67+) and differentiating (Doublecortin+) cells. Dcx, doublecortin. Scale bars, 10um and 5um. WT, wild-type.