Sensory deprivation increased layer 2/3 pyramidal neuron spontaneous firing. A, Examples of spontaneous firing from layer 2/3 pyramidal neurons from control (black), LS (dark gray), and TTX (light gray) conditions. B, Bar plot showing average spontaneous firing frequency for each condition. Data from slices ipsilateral to the sutured eye (control LS; black bar), contralateral to the sutured eye (LS; white bar), ipsilateral to the TTX-injected eye (control TTX; dark gray bar), and contralateral to the TTX-injected eye (TTX; light gray bar) are shown. *Significantly different from control.