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. Author manuscript; available in PMC: 2009 Mar 13.
Published in final edited form as: J Biol Chem. 2005 Jun 30;280(34):30214–30224. doi: 10.1074/jbc.M506103200

Fig. 9. Purified 1-phosphomethyl-lipid A does not activate TLR2-expressing HEK cells.

Fig. 9

HEK293 cells expressing TLR2YFP (squares), TLR4YFP/MD2 (triangles), or empty vector (diamonds) were stimulated with increasing concentrations of 1-phosphomethyl-lipid A (A) or unmodified lipid A (B). LPS (10 ng/ml), Pam2CysK4 (2.5 nM), and recombinant human IL-1β (10 ng/ml) were used as positive controls (C). Cellular activation was measured by luciferase activity.