Figure 1.

Nbl1p is part of the CPC. (A) Confirmation of NBL1 expression by RT-PCR using duplicate cDNA samples. gDNA, genomic DNA (B) Growth phenotype of nbl1-6. Serial 10-fold dilutions of NBL1 and nbl1-6 cells on synthetic minimal medium plates incubated at 25 or 37°C for 48 h. (C) Nbl1p copurifies with the other CPC components. Sli15-TAP and Nbl1-TAP purified complexes. Top, silver-stained 12% discontinuous gel. Note that Nbl1p is too small to be seen on a discontinuous gel that is able to resolve Sli15p and Bir1p. ^† Bands that always copurify with Sli15-TAP (Cheeseman et al., 2002). * Contaminants. Bottom, Western blot of 4–20% continuous gel.