Figure 6.

Nbl1p is a highly diverged homolog of Borealin/Dasra/CSC-1. (A) Block diagram summarizing features of sequences similar to Borealin/Dasra/CSC-1 from various fungal and animal taxa. Frequency of amino acid occurrence in conserved regions from the protein sequence alignment are shown as sequence logos (WebLogo: http://weblogo.berkeley.edu/; Crooks et al., 2004). (B) Phylogeny of Borealin/Dasra/CSC-1–related sequences. Consensus Parsimony tree with branch lengths estimated by Maximum Likelihood using PHYLIP v. 3.68 (http://evolution.genetics.washington.edu/phylip.html). Default settings were used and the sequence order was jumbled 10 times for the 1000 bootstrap replicates. The tree was based on the THB-forming domains from proteins listed in Supplemental Table S2A. Protein sequences missing more than 10% of this region were excluded. Borealin/Dasra/CSC-1–related sequences that have been characterized are underlined.